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Development of functional neuronal networks

Juan Burrone

Juan Burrone

Professor of Developmental Neurophysiology

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Overview

Neurons in the brain receive a large number of synaptic inputs along their vast dendritic trees. These inputs are not only highly heterogeneous, they also have different identities that can either excite or inhibit neurons, providing an added level of complexity. The information carried by these inputs is subsequently integrated to produce an output, the action potential, which is elicited at a specific location along the axon of a neuron, known as the axon initial segment (AIS). We are interested in understanding the process whereby a neuron integrates synaptic inputs to generate an output. Our questions range from establishing how synaptic inputs form and distribute along dendrites, to how the distribution of excitatory and inhibitory inputs impacts on the output of a neuron and how both inputs (synapses) and outputs (AIS) are modified by neuronal or circuit activity to stabilise the network. Our lab focuses on three main aspects of neuronal and circuit function: (i) understanding synaptic transmission and integration; (ii) studying how synapses form and mature and (iii) exploring how neurons and circuits control their excitability through homeostatic forms of plasticity. We use a combination of imaging, serial block-face scanning electron microscopy, electrophysiology and molecular techniques to approach these subjects. We take a bottom-up approach, where rules learnt in simpler in vitro systems, such as dissociated hippocampal/cortical neurons and organotypic brain slices are taken to more intact systems, such as acute brain slices and the in vivo brain.

Selected publications

Gonzalez Sabater V, Rigby M, Burrone J (2021) Voltage-gated potassium channels ensure action potential shape fidelity in distal axons. J Neurosci

Pan-Vazquez A, Wefelmeyer W, Gonzalez Sabater V, Neves G, Burrone J (2020) Activity-Dependent Plasticity of Axo-axonic Synapses at the Axon Initial Segment. Neuron 106: 265-276.e6

Grillo FW, Neves G, Walker A, Vizcay-Barrena G, Fleck RA, Branco T, Burrone J (2018) A Distance-Dependent Distribution of Presynaptic Boutons Tunes Frequency-Dependent Dendritic Integration. Neuron 99: 275-282.e3

Denaxa M, Neves G, Rabinowitz A, Kemlo S, Liodis P, Burrone J, Pachnis V (2018) Modulation of Apoptosis Controls Inhibitory Interneuron Number in the Cortex. Cell Rep 22: 1710-1721

Andreae LC, Burrone J (2015) Spontaneous Neurotransmitter Release Shapes Dendritic Arbors via Long-Range Activation of NMDA Receptors. Cell Rep 15: S2211-1247