Our experimental approach
We believe that understanding brain development will depend on uncovering how individual cells contribute to brain morphogenesis and how the subcellular organisation of cells drives their behaviours. For these reasons we use the transparency and accessibility of the living zebrafish embryo brain together with advanced in vivo microscopy to reveal cell and sub-cellular behaviours. We recently developed an optogenetic approach that uses far red laser light to manipulate sub-cellular protein localisation with high spatial and temporal precision in the intact embryo. This should allow more precise dissection of cell polarity and cellular asymmetries in vivo.