Synaptic transmission and integration
We are interested in understanding the process of information transfer at the chemical synapse and its relation to neuronal output. A large part of our work in this area focuses on understanding how neurotransmitter is released at individual presynaptic boutons and how they subsequently activate postsynaptic receptors. To tackle these questions we make use of fluorescent genetically-encoded probes that provide a functional readout of calcium, vesicle recycling, glutamate release and voltage, together with super-resolution microscopy to probe synapse structure at the nanoscale. Our aim is to combine the highly sensitive synaptic reporters currently available to characterize pre- and postsynaptic function at the level of a single synapse and, more globally, along an entire dendritic structure. Together with readouts of synaptic output (elecrohpysiology and voltage indicators), we hope to understand how the spatiotemporal integration of synaptic inputs onto a neuron controls its output.