Genome Editing


Genome Editing
Since 2011, the CDN has a team of two research scientists, funded by the Wellcome Trust, who developed a centralised genome editing facility, using TALE nuclease and CRISPR/Cas9 technology to generate targeted knock-out and knock-in mutants in zebrafish. The aims of this facility are threefold: i) creating a technical platform providing high-throughput mutations (80 genes in three years) in genes coding for key players in neurobiology; ii) make the TALEN and CRISPR tools produced available to the research community and ii) improving the technology by developing TALEN-induced homologous recombination in vivo, thereby building conditional loss of function tools in zebrafish. Moreover, our staff is training scientists around the world (to date UK, Italian, Israeli and French scientists). By 2015, we will expand the tasks of the facility to genome editing in mouse and human ES/iPS cells.